Avaliação morfológica após o uso do ozônio gasoso via intra articular em ratos com osteoartrose induzida por monoiodoacetato de sódio

Abstract

Aging is responsible for a series of physiological changes that affect the skeletal muscle system in the elderly, including osteoarthritis (OA) of the knee. This pathology is progressive and can cause pain and limitations in activities of daily living. It mainly affects the elderly and it increasingly becomes a public health problem, due to the limited ability to repair cartilage tissue. The objective of this dissertation is to evaluate the effects of intra-articular ozone gas injection in the knee of rats with sodium monoiodoacetate (MIA) induced OA on the articular cartilage morphology. This is an experimental study, with approval by the Ethics Committee on the Use of Animals (CEUA) of the University of Passo Fundo (UPF) under Protocol No. 003/2019 and financed with support from FAPERGS - Brazil (FAPERGS) through the Announcement ARD 2019. 40 male and female Wistar rats aged 12 weeks provided by the Vivarium of the University of Passo Fundo (UPF - Passo Fundo, RS, Brazil) were used. The animals were randomly divided into 4 groups: G1 (control); G2 (injury); G3 (low dose ozone treatment); G4 (high dose ozone treatment). The present study was necessary to evaluate the effects of ozone gas on the knee joint cartilage in rats with OA. The lesion was chemically induced with an intra-articular injection with MIA, after the diagnosis of OA, the treatment with intra-articular ozone gas was performed. For statistical analysis, the results were expressed as mean ± standard deviation. The data were submitted to the normality test by the D’Agostino Pearson test, normally distributed using ANOVA with post test Tuckey and, for non-normal data Kruskall Wallis followed by Dunn’s post test. Values considered significant with p <0.05. The density of tibial joint volume **** G1 / G2 (p <0.0001), *** G1 / G3 (p <0.0008), ** G1 / G4 (p <0.0012), # ### G2 / G3 (p <0.0001), #### G2 / G4 (p <0.0001). And for the femur **** G1 / G2 (p <0.0001), #### G2 / G3 (p <0.0001), #### G2 / G4 (p <0.0001). Conclusion: ozone has positive anti-inflammatory effects mainly on clinical symptoms, the present study demonstrates that the use of ozone can delay the degeneration of articular cartilage.