Avaliação da eficácia antimicrobiana de instrumentos reciprocantes e uma substância química experimental

Abstract

The major goal of the endodontic therapy is the reduction or elimination of microorganisms present in the root canals. To achieve this objective, the association of physical action of endodontic instruments with the chemical action of auxiliary substances is required, performing a chemical and mechanical preparation of the root canal. This study aimed to evaluate the reduction in bacterial levels obtained by different techniques of instrumentation and auxiliary chemicals. To determine the antimicrobial effectiveness of reciprocating instruments, 50 mesiobuccal roots of maxillary molars were contaminated with Enterecoccus faecalis and divided into 4 groups: G1 (n=5), sterile roots with no contamination (negative control); G2 (n=5) contaminated roots (positive control); G3 (n=20) root preparation with Reciproc R25; and G4 (n=20) preparation with Reciproc R25 associated with the instrument Mtwo 40.04. The potential of decontamination of an experimental chemical irrigant was determined by using 50 distobuccal roots from the same teeth. Roots were contaminated with E. faecalis and divided into 6 groups: G1 (n= 5) negative control; G2 (n= 5) positive control; G3 (n= 10) 5.25% sodium hypochlorite (NaOCl) + 17% EDTA; G4 (n= 10) 2% Chlorhexidine (CHX) gel and physiologic solution + 17% EDTA; and G5 (n= 10) 6.5% Grape Seed Extract