Ficocianina na proteção da toxicidade da alfa-synucleína e deleção do gene SIR2 em Saccharomyces cerevisiae

Abstract

The increase in oxidative stress levels influence the Parkinson's disease is a neurodegenerative disorder of aging in higher risk. The formation of protein aggregates of alpha-Parkinsonian synucleína (αSyn) leads to generating frames of toxicity key event in the progression of the disease. The need for new therapies that can control and prevent damage caused by oxidative stress becomes important and this capacity can result from the use of natural functional substances. Our study examined the protective effect of phycocyanin, the main pigment of Spirulina platensis in Saccharomyces cerevisiae model expression for the αSyn protein and deletion of SIR2 gene (ΔSIR2). The effect of phycocyanin was analyzed by parameters of lipid peroxidation, enzymatic activity of catalase, glutathione and superoxide dismutase and also in cell viability, represented by assays in triplicate and analyzed using ANOVA and Tukey's test. Our results showed that the expression of phycocyanin opposite αSyn and deletion of SIR2 gene significantly increases the enzymatic activity of CAT and GPX maintains SOD levels, increases cell viability and decreases the levels of lipid peroxidation. The phycocyanin introduced us to be a promising functional substance for the investigation of new therapies against damage caused by oxidative stress in neurodegenerative diseases mainly for prevention of Parkinson's disease